Antioxidant activity by dpph method pdf

The insects and juvenile hormone mimicking activity. Leaf juice is used as Scientific evidence suggests that antioxidants reduce risk for galactagogue. Poultice of the herb is applied to sores, boils and to chronic diseases including cancer and heart disease. Most of the relieve stomachache. The main characteristic of an pectic acids, gum, minerals and their salts. Highly reactive free radicals and oxygen species are present in biological systems from a wide variety of sources.

The DPPH method is described as a simple, rapid and convenient method independent of sample polarity for screening of many samples for radical scavenging activity.

Kalmegh Andrographis paniculata Nees, king of bitter or Moha- Test plant materials tita family: Acanthaceae is an important and well known medicinal Fresh samples were collected from the Medicinal germplasm plant in the world.

In Bangladesh, there are more than 20 species and horsetail Equisetum debile , leaf of Iron-wood tree Memecylon 8 varieties of the genus Andrographis available,17 including A. Only a few species are medicinal, of which A.

Since ancient times, Kalmegh is used in and leaf of Kalmegh Andrographis paniculata were taken for the traditional Siddha and Ayurvedic systems of medicine as well as in investigation. The details of tested plant materials are presented in tribal medicine in India and some other countries for multiple clinical Table 1. Antimicrobial and antioxidant activity analysis of some medicinal plants of Bangladesh. Adv Plants Agric Res. DOI: Then the samples were blended by a blender machine Moulinex, France.

Dried powders of different treatments were Test organisms dissolved in sample bottles with different solvents for 3days. Each day each bottle was shacked well and filtered with a filter paper Clinical isolates of the microorganisms were used for antimicrobial Millipore, Germany. The filtrate was then evaporated by a rotary test Table 2. The pathogenic bacterial and fungal pure cultures were vacuum evaporator Buchi, England.

This procedure was repeated collected from the Institute of Nutrition and Food Science INFS , for times until the total compounds separated from the powder. All University of Dhaka, Bangladesh. Both Gram positive and Gram- evaporations were carried out under reduced pressure using a rotary negative organisms were taken in this experiment.

UV light was switched on one hour before working in the Laminar Hood. In an aseptic condition under laminar air cabinet, the yeast extract The make the required volume. The test tubes were then capped and sterilized by autoclaving at lbs Preparation of the test plates pressuresq. The slants were used for making fresh culture of bacteria and fungi that were in turn used for The test organisms were transferred from the subculture to the test sensitivity study.

In order to avoid any type of contamination and tubes containing about 10ml of melted and sterilized agar medium cross contamination by the test organisms the antimicrobial screening with the help of a sterilized transfer loop in an aseptic area.

The bacterial and fungal suspension was immediately the available methods, disc diffusion method for antimicrobial assay transferred to the sterilized petri-dishes. The petri-dishes were rotated and on the other hand, the compounds distributed in plants have the several times clockwise and anticlockwise to assure homogenous ability to scavenge free radicals by single-electron transfer.

In our distribution of the test organisms in the media. Because of the complex nature of Preparation of sample discs with test samples phytochemicals, the antioxidant and antimicrobial activities of plant Test samples were dissolved in solvent to obtain the desired extracts should be evaluated separately.

The results of this experiment concentrations in an aseptic condition. Sterilized metrical filter paper would be encouraged in future to the application of these medicinal discs BBL, Cocksville, USA were taken in a blank petridish and plants in pharmaceutical and cosmetic formulations.

The sample discs, standard antibiotic discs and control discs were placed gently on the previously marked extracts zones in the agar plates pre-inoculated with test bacteria and fungi. The plates were then inverted and kept fungi Table 2. The crude MeOH extract inhibition of the dry leaves of P. The antimicrobial activity of crude MeOH extract of A. The absorbance values were measured after Salmonella and or other organisms Table 3. EA extract showed an The free radical scavenging activity of samples was calculated excellent inhibition activity 15mm found by gram positive Bacillus according to the formula.

DPPH solution 0. However, the n-hexane extracts did not show any activity against The ascorbic acid vitamin C was used as a positive control. Each the bacteria, fungi and yeast. The microbial activity was remarkable treatment was replicated twice.

Antioxidant activity was expressed as in the seeds of D. The butanol extracts of A. Among mm at a concentration of mg disc-1 Table 3. A microorganisms. The antimicrobial activity of MeOH extract of M. The results showed moderate antimicrobial activity all the used extracts exhibited the various scavenging capacity. The mm against all organisms except Sarcina lutea Table 3.

The best free radical scavenging activity of the seventeen different extracts antimicrobial activity was noticed against Bacillus megaterium. The MeOH extract of A. Bacillus subtilis that red kidney beans had higher activity than other beans. Salmonella paratyphi, Vibrio spp. In medicinal plant samples an experiment, Kumar et al. Methanol 11 10 10 9 10 10 10 10 10 9 9 10 10 10 10 10 Guianensis A.

Ghani A. Medicinal Plants of Bangladesh. Screening for antibacterial activity B4 Ethn Leaflets. Veprisinium salt, a novel antibacterial quaternary alkaloid from Vepris louisii. Planta Med. B7 B8 Oxygen-radical absorbance capacity assay for antioxidants. Chevion, S. The use of cyclic voltammetry for the evaluation of antioxidant capacity. Christopoulos, A.

Assessing the distribution of parameters in models of 4. Conclusions ligand—receptor interaction: To log or not to log. Trends in Pharmacological Sciences, 19 9 , — These findings underscore the importance of an appropriate Eklund, P.

Antioxidant activity of pomegranate juice and its relationship with phenolic present study is focused on the definition of the most accurate sta- composition and processing. Journal of Agricultural and Food Chemistry, 48 10 , tistical program for EC50 determination, which is generally used as — Phycoerythrin fluorescence-based assay for reactive oxygen species. Methods in Enzymology, , — In the present research, we performed experimental Knudsen, L.

The use of the angular transformation in and statistical studies using single antioxidant compounds since biological assays. Journal of the American Statistical Association, 42 , the implications are conveyable to mixtures of compounds such — Krishnaiah, D. A review of the antioxidant as plant extracts and foods. Food and Bioproducts Processing, 89 3 , GraphPad Prism is a flexible and worldwide used software for ba- — Study of the DPPH center dot-scavenging activity: Development of a free exclusively developed for biological and pharmacological use.

Food Chemistry, 3 , — Among the programs considered in the present study, GraphPad Miller, N. A novel Prism 5P was the only that could easily load all datasets together method for measuring antioxidant capacity and its application to monitoring and could plot them on the same graphic, with an automatic update the antioxidant status in premature neonates.

Clinical Science, 84 4 , — Mishra, K. Estimation of antiradical properties after every data changing in the spreadsheet cells. Food Chemistry, presented above, we suggest the five-parameter regression model , — Fitting curves to data using nonlinear Sharma, O.

DPPH antioxidant assay revisited. Food Chemistry, regression: A practical and nonmathematical review. Tabart, J. Comparative Nenadis, N.

Use of reference compounds in antioxidant capacities of phenolic compounds measured by various tests. Food antioxidant activity assessment. Journal of Agricultural and Food Chemistry, Chemistry, 4 , — Valko, M.

Free Norusis, M. SPSS Upper Saddle River, NJ: radicals and antioxidants in normal physiological functions and human disease. Prentice Hall. Prior, R. Standardized methods for the Van der Graaf, P.

Analysis of asymmetry of agonist determination of antioxidant capacity and phenolics in foods and dietary concentration—effect curves. Journal of Pharmacological and Toxicological supplements. Journal of Agricultural and Food Chemistry, 53 10 , — Methods, 41 2—3 , — Scalbert, A. Polyphenols: Antioxidants and Villano, D. Comparison of antioxidant activity of wine phenolic compounds and metabolites in vitro. Analytica Chimica Acta, 1—2 , — The antimicrobial activity of MeOH extract of M.

The results showed moderate antimicrobial activity all the used extracts exhibited the various scavenging capacity. The mm against all organisms except Sarcina lutea Table 3. The best free radical scavenging activity of the seventeen different extracts antimicrobial activity was noticed against Bacillus megaterium. The MeOH extract of A. Bacillus subtilis that red kidney beans had higher activity than other beans. Salmonella paratyphi, Vibrio spp. In medicinal plant samples an experiment, Kumar et al.

Methanol 11 10 10 9 10 10 10 10 10 9 9 10 10 10 10 10 Guianensis A. Ghani A. Medicinal Plants of Bangladesh. Screening for antibacterial activity B4 Ethn Leaflets. Veprisinium salt, a novel antibacterial quaternary alkaloid from Vepris louisii. Planta Med. B7 B8 Food Chem. B9 Sanchez—Moreno C. Review:Methods used to evaluate the free radical B10 Food Sci Technol Intl.

B11 Investigation of plant extracts B12 Comparison of antioxidant assays based on radical scavenging, lipid oxidation and B13 Eur Food Res Technol. B14 J Ethnopharmacol. B16 Phytochemistry and pharmacological B17 Int J Pharm Phytopharmacol Res. Evaluation of antiulcer activity experiments. Asian J Pharm Tech. Lim TK. Couroupita guianensis. Ratsch C. Int J Pharm Tech Res. Screening of antimicrobial potential and phytoconsituents for different extractsof Memecylon umbellatum Burm Conclusion inflorescences.

Asian J Pharm Res. From the above result it can be concluded that different plant Antimicrobial potential and phytochemical extract had shown significant antimicrobial and antioxidant screening of different bark extracts of Memecylon umbellatum Burm. Int J properties. Among the samples, ethyl acetate extract of Aloe vera Appl Microbiol Sci. Surprisingly Phytochemical screening and in— petroleum ether extract of Datura seed showed remarkable microbial vitro antioxidant activity of Memecylon umbellatum root extracts.

World J activity against the bacteria and viruses and it can be concluded that Pharm Pharm Sci. Wound healing activity of medicine and cosmetic industry. Cyperus rotundus Linn. Indian J Pharm Sci. Chavellier A. The Encyclopedia of Medicinal Plants. New York, Blois MS. Antioxidant determination by the use of a stable free radical. Cytotoxic, antioxidant Scavenging effect of methanolic extracts of peanut and antibacterial activities of Varthemia iphionoides Boiss.

J hulls on free—radical and active—oxygen species. J Agric Food Chem. Effect of Organic Effect of probiotic combination Fertilizers and Spacing on Growth and Yield of Kalmegh Andrographis fermented soybean meal on growth performance, lipid metabolism and paniculata Nees.

Intl J Agric Crop Sci. Asian J Anim Vet Adv. Testicular toxicity assessment of Andrographis paniculata dried extract in rats. Engineering the gram positive ;58 3 — Foster S. Aloe vera: The succulent with skin soothing cell—protecting properties Herbs for Health magazine.